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am580 retinoic acid agonist  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc am580 retinoic acid agonist
    Am580 Retinoic Acid Agonist, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/am580+retinoic+acid+agonist/am580+retinoic+acid+agonist/pmc12066771-64-32-37
    Average 90 stars, based on 1 article reviews
    am580 retinoic acid agonist - by Bioz Stars, 2026-10
    90/100 stars

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    Cell Culture:

    Article Title: Mitochondrial metabolism is rapidly re-activated in mature neutrophils to support stimulation-induced response
    Article Snippet: .. Following collection, progenitor cells were cultured in StemSpan SFEM II medium (STEMCELL Technologies), supplemented with GlutaMAX 100× (1×; Thermo Fisher Scientific), ExCyte 0.2% (Merck Millipore), human granulocyte colony-stimulating factor (150ng/mL; PeproTech), and Am580 retinoic acid agonist (2.5μM; STEMCELL Technologies) at 0.8–1 × 10 6 cells/mL density. ..

    Article Title: Neutrophil motility is regulated by both cell intrinsic and endothelial cell ARPC1B
    Article Snippet: .. These cells were cultured in StemSpan SFEM II medium (STEMCELL Technologies), supplemented with GlutaMAX 100X (Thermo Fisher Scientific), ExCyte 0.2% (Merck Millipore), human G-CSF (150 ng/mL; PeproTech), and Am580 retinoic acid agonist 2.5 μM (STEMCELL Technologies) at 2 × 10 6 cells/mL density. ..



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    Expression of ANKRD55 and neighboring genes in immature moDC. (A–D) Monocytes were cultivated for 6 days in IL-4/GM-CSF medium for differentiation into immature moDC in the absence (colored curves) or presence (gray curves) of AM-580, and expression levels of the indicated genes were measured by qPCR at the start of the cultivation (day 0) and on days 1, 2, 4, and 6. Mean ± SEM of 3 independent measurements; Friedman test (followed by Dunn’s multiple comparison test) for comparison of data points in each curve with day 0 and Wilcoxon test for comparison of both curves (Control vs. <t>AM580).</t> (E–H) Effect of tolerogenic compounds on gene expression in immature moDC by qPCR. Concentrations of compounds are provided in the Materials and Methods . Mean ± SEM of maximum 5 independent measurements; Friedman test (followed by Dunn’s multiple comparison test). (I) Analysis of ALDH1A2, <t>RARα,</t> and RARγ expression by qPCR. Mean ± SEM; n = 6; Friedman test (followed by Dunn’s multiple comparison test). (J) Expression levels of three discrete ANKRD55 splice variants 201, 202, and 204 in immature moDC. Mean ± SEM; n = 10; Wilcoxon test. (K) Classical (Cla; CD14 hi /CD16 - ), intermediate (Int; CD14 hi /CD16 + ), and non-classical (NC; CD14 + /CD16 + ) monocytes were isolated and separately cultivated for 6 days to differentiate into moDC. Gene expression levels were measured in the original monocyte subsets (M) and derived moDC (D). Mean ± SEM; n = 3; Mann–Whitney test for comparison between monocytes and moDC for each gene and subset. * p ≤ 0.05, ** p ≤ 0.01.
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    Expression of ANKRD55 and neighboring genes in immature moDC. (A–D) Monocytes were cultivated for 6 days in IL-4/GM-CSF medium for differentiation into immature moDC in the absence (colored curves) or presence (gray curves) of AM-580, and expression levels of the indicated genes were measured by qPCR at the start of the cultivation (day 0) and on days 1, 2, 4, and 6. Mean ± SEM of 3 independent measurements; Friedman test (followed by Dunn’s multiple comparison test) for comparison of data points in each curve with day 0 and Wilcoxon test for comparison of both curves (Control vs. <t>AM580).</t> (E–H) Effect of tolerogenic compounds on gene expression in immature moDC by qPCR. Concentrations of compounds are provided in the Materials and Methods . Mean ± SEM of maximum 5 independent measurements; Friedman test (followed by Dunn’s multiple comparison test). (I) Analysis of ALDH1A2, <t>RARα,</t> and RARγ expression by qPCR. Mean ± SEM; n = 6; Friedman test (followed by Dunn’s multiple comparison test). (J) Expression levels of three discrete ANKRD55 splice variants 201, 202, and 204 in immature moDC. Mean ± SEM; n = 10; Wilcoxon test. (K) Classical (Cla; CD14 hi /CD16 - ), intermediate (Int; CD14 hi /CD16 + ), and non-classical (NC; CD14 + /CD16 + ) monocytes were isolated and separately cultivated for 6 days to differentiate into moDC. Gene expression levels were measured in the original monocyte subsets (M) and derived moDC (D). Mean ± SEM; n = 3; Mann–Whitney test for comparison between monocytes and moDC for each gene and subset. * p ≤ 0.05, ** p ≤ 0.01.
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    Expression of ANKRD55 and neighboring genes in immature moDC. (A–D) Monocytes were cultivated for 6 days in IL-4/GM-CSF medium for differentiation into immature moDC in the absence (colored curves) or presence (gray curves) of AM-580, and expression levels of the indicated genes were measured by qPCR at the start of the cultivation (day 0) and on days 1, 2, 4, and 6. Mean ± SEM of 3 independent measurements; Friedman test (followed by Dunn’s multiple comparison test) for comparison of data points in each curve with day 0 and Wilcoxon test for comparison of both curves (Control vs. AM580). (E–H) Effect of tolerogenic compounds on gene expression in immature moDC by qPCR. Concentrations of compounds are provided in the Materials and Methods . Mean ± SEM of maximum 5 independent measurements; Friedman test (followed by Dunn’s multiple comparison test). (I) Analysis of ALDH1A2, RARα, and RARγ expression by qPCR. Mean ± SEM; n = 6; Friedman test (followed by Dunn’s multiple comparison test). (J) Expression levels of three discrete ANKRD55 splice variants 201, 202, and 204 in immature moDC. Mean ± SEM; n = 10; Wilcoxon test. (K) Classical (Cla; CD14 hi /CD16 - ), intermediate (Int; CD14 hi /CD16 + ), and non-classical (NC; CD14 + /CD16 + ) monocytes were isolated and separately cultivated for 6 days to differentiate into moDC. Gene expression levels were measured in the original monocyte subsets (M) and derived moDC (D). Mean ± SEM; n = 3; Mann–Whitney test for comparison between monocytes and moDC for each gene and subset. * p ≤ 0.05, ** p ≤ 0.01.

    Journal: Frontiers in Immunology

    Article Title: Genomic Multiple Sclerosis Risk Variants Modulate the Expression of the ANKRD55 – IL6ST Gene Region in Immature Dendritic Cells

    doi: 10.3389/fimmu.2021.816930

    Figure Lengend Snippet: Expression of ANKRD55 and neighboring genes in immature moDC. (A–D) Monocytes were cultivated for 6 days in IL-4/GM-CSF medium for differentiation into immature moDC in the absence (colored curves) or presence (gray curves) of AM-580, and expression levels of the indicated genes were measured by qPCR at the start of the cultivation (day 0) and on days 1, 2, 4, and 6. Mean ± SEM of 3 independent measurements; Friedman test (followed by Dunn’s multiple comparison test) for comparison of data points in each curve with day 0 and Wilcoxon test for comparison of both curves (Control vs. AM580). (E–H) Effect of tolerogenic compounds on gene expression in immature moDC by qPCR. Concentrations of compounds are provided in the Materials and Methods . Mean ± SEM of maximum 5 independent measurements; Friedman test (followed by Dunn’s multiple comparison test). (I) Analysis of ALDH1A2, RARα, and RARγ expression by qPCR. Mean ± SEM; n = 6; Friedman test (followed by Dunn’s multiple comparison test). (J) Expression levels of three discrete ANKRD55 splice variants 201, 202, and 204 in immature moDC. Mean ± SEM; n = 10; Wilcoxon test. (K) Classical (Cla; CD14 hi /CD16 - ), intermediate (Int; CD14 hi /CD16 + ), and non-classical (NC; CD14 + /CD16 + ) monocytes were isolated and separately cultivated for 6 days to differentiate into moDC. Gene expression levels were measured in the original monocyte subsets (M) and derived moDC (D). Mean ± SEM; n = 3; Mann–Whitney test for comparison between monocytes and moDC for each gene and subset. * p ≤ 0.05, ** p ≤ 0.01.

    Article Snippet: To induce tolerogenicity, DCs were treated during the differentiation process with 100 nM retinoic acid receptor-α agonist AM580 (Sigma-Aldrich, Ref. A8843), 10 nM vitamin D3 (STEMCELL Technologies, Ref. 72412), 1 μM prostaglandin E2 (PGE2) (Sigma-Aldrich, Ref. P0409), 20 ng/ml IL-10 (Peprotech, Ref. AF-200-10), 1 μM dexamethasone (Sigma-Aldrich, Ref. D4902), and 10 ng/ml rapamycin (STEMCELL Technologies, Ref. 73362), and all were added on day 0, and again on day 3, of the moDC differentiation period.

    Techniques: Expressing, Isolation, Derivative Assay, MANN-WHITNEY

    Effect of maturation of moDC on the expression of ANKRD55 , IL6ST , IL31RA , and SLC38A9 . (A) Comparison of the effect of maturation by CpG, poly(I:C)/LPS and IFN-γ/LPS on expression levels of the main ANKRD55 splice variants by qPCR. Mean ± SEM; n = 3; Friedman test (followed by Dunn’s multiple comparison test). (B) Flow cytometry to assess the maturation percentage induced by the indicated stimuli based on the expression of CD209 (expressed in moDC) and CD83 (expressed in mature moDC only). Shown is a representative experiment out of 4 performed. (C–F) MoDCs cultivated for 5 days in MoDC medium were matured by addition of IFN-γ and 24 h later LPS. Gene expression was analyzed by qPCR coinciding with time of IFN-γ (day 5) and LPS (0 h) addition and 3, 6, 12, 24, and 48 h later. The experiment was also performed in cells differentiated in the presence of AM-580 (gray curves). Mean ± SEM of 3 independent measurements; Friedman test (followed by Dunn’s multiple comparison test) for comparison of data points in each curve with day 5 and Wilcoxon test for comparison of both curves (control vs. AM580). * p ≤ 0.05, ** p ≤ 0.01.

    Journal: Frontiers in Immunology

    Article Title: Genomic Multiple Sclerosis Risk Variants Modulate the Expression of the ANKRD55 – IL6ST Gene Region in Immature Dendritic Cells

    doi: 10.3389/fimmu.2021.816930

    Figure Lengend Snippet: Effect of maturation of moDC on the expression of ANKRD55 , IL6ST , IL31RA , and SLC38A9 . (A) Comparison of the effect of maturation by CpG, poly(I:C)/LPS and IFN-γ/LPS on expression levels of the main ANKRD55 splice variants by qPCR. Mean ± SEM; n = 3; Friedman test (followed by Dunn’s multiple comparison test). (B) Flow cytometry to assess the maturation percentage induced by the indicated stimuli based on the expression of CD209 (expressed in moDC) and CD83 (expressed in mature moDC only). Shown is a representative experiment out of 4 performed. (C–F) MoDCs cultivated for 5 days in MoDC medium were matured by addition of IFN-γ and 24 h later LPS. Gene expression was analyzed by qPCR coinciding with time of IFN-γ (day 5) and LPS (0 h) addition and 3, 6, 12, 24, and 48 h later. The experiment was also performed in cells differentiated in the presence of AM-580 (gray curves). Mean ± SEM of 3 independent measurements; Friedman test (followed by Dunn’s multiple comparison test) for comparison of data points in each curve with day 5 and Wilcoxon test for comparison of both curves (control vs. AM580). * p ≤ 0.05, ** p ≤ 0.01.

    Article Snippet: To induce tolerogenicity, DCs were treated during the differentiation process with 100 nM retinoic acid receptor-α agonist AM580 (Sigma-Aldrich, Ref. A8843), 10 nM vitamin D3 (STEMCELL Technologies, Ref. 72412), 1 μM prostaglandin E2 (PGE2) (Sigma-Aldrich, Ref. P0409), 20 ng/ml IL-10 (Peprotech, Ref. AF-200-10), 1 μM dexamethasone (Sigma-Aldrich, Ref. D4902), and 10 ng/ml rapamycin (STEMCELL Technologies, Ref. 73362), and all were added on day 0, and again on day 3, of the moDC differentiation period.

    Techniques: Expressing, Flow Cytometry

    Localization of ANKRD55 in (A) monocytes (1.024 × 1.024 pixels = 0.131 microns/pixel) and (B–E) moDC by immunofluorescence microscopy. Rightmost graph represents mean ± SEM in monocytes and immature moDC ( n = 5 cellular ROIs/group; unpaired t -test). (B) Colocalization of ANKRD55 with CD209, a membrane marker specific for moDC (2.048 × 2.048 pixels = 0.065 microns/pixel). (C) Effect of AM580 and IFN-γ/LPS treatment on nuclear ANKRD55 signal in immunofluorescence (1,808 × 1,808 pixels = 0,074 microns/pixel). The diagram on the right provides quantitative analysis of nuclear ANKRD55 immunofluorescence [mean ± SEM; n ≥ 59 cellular ROIs/condition; Kruskal–Wallis test (followed by Dunn’s multiple comparison test)]. (D) Colocalization of ANKRD55 with ALYREF (1.024 × 1.024 pixels = 0.131 microns/pixel). (E) Colocalization of ANKRD55 with HNRNPC (1.024 × 1.024 pixels = 0.131 microns/pixel). *** p ≤ 0.001, **** p ≤ 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Genomic Multiple Sclerosis Risk Variants Modulate the Expression of the ANKRD55 – IL6ST Gene Region in Immature Dendritic Cells

    doi: 10.3389/fimmu.2021.816930

    Figure Lengend Snippet: Localization of ANKRD55 in (A) monocytes (1.024 × 1.024 pixels = 0.131 microns/pixel) and (B–E) moDC by immunofluorescence microscopy. Rightmost graph represents mean ± SEM in monocytes and immature moDC ( n = 5 cellular ROIs/group; unpaired t -test). (B) Colocalization of ANKRD55 with CD209, a membrane marker specific for moDC (2.048 × 2.048 pixels = 0.065 microns/pixel). (C) Effect of AM580 and IFN-γ/LPS treatment on nuclear ANKRD55 signal in immunofluorescence (1,808 × 1,808 pixels = 0,074 microns/pixel). The diagram on the right provides quantitative analysis of nuclear ANKRD55 immunofluorescence [mean ± SEM; n ≥ 59 cellular ROIs/condition; Kruskal–Wallis test (followed by Dunn’s multiple comparison test)]. (D) Colocalization of ANKRD55 with ALYREF (1.024 × 1.024 pixels = 0.131 microns/pixel). (E) Colocalization of ANKRD55 with HNRNPC (1.024 × 1.024 pixels = 0.131 microns/pixel). *** p ≤ 0.001, **** p ≤ 0.0001.

    Article Snippet: To induce tolerogenicity, DCs were treated during the differentiation process with 100 nM retinoic acid receptor-α agonist AM580 (Sigma-Aldrich, Ref. A8843), 10 nM vitamin D3 (STEMCELL Technologies, Ref. 72412), 1 μM prostaglandin E2 (PGE2) (Sigma-Aldrich, Ref. P0409), 20 ng/ml IL-10 (Peprotech, Ref. AF-200-10), 1 μM dexamethasone (Sigma-Aldrich, Ref. D4902), and 10 ng/ml rapamycin (STEMCELL Technologies, Ref. 73362), and all were added on day 0, and again on day 3, of the moDC differentiation period.

    Techniques: Immunofluorescence, Microscopy, Marker

    p -Values corresponding to the group-by-time interaction term’s significance in the adjusted generalized linear mixed univariable models.

    Journal: Frontiers in Immunology

    Article Title: Genomic Multiple Sclerosis Risk Variants Modulate the Expression of the ANKRD55 – IL6ST Gene Region in Immature Dendritic Cells

    doi: 10.3389/fimmu.2021.816930

    Figure Lengend Snippet: p -Values corresponding to the group-by-time interaction term’s significance in the adjusted generalized linear mixed univariable models.

    Article Snippet: To induce tolerogenicity, DCs were treated during the differentiation process with 100 nM retinoic acid receptor-α agonist AM580 (Sigma-Aldrich, Ref. A8843), 10 nM vitamin D3 (STEMCELL Technologies, Ref. 72412), 1 μM prostaglandin E2 (PGE2) (Sigma-Aldrich, Ref. P0409), 20 ng/ml IL-10 (Peprotech, Ref. AF-200-10), 1 μM dexamethasone (Sigma-Aldrich, Ref. D4902), and 10 ng/ml rapamycin (STEMCELL Technologies, Ref. 73362), and all were added on day 0, and again on day 3, of the moDC differentiation period.

    Techniques: